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Publications and Patents

Liquid–liquid phase separation of lamin drives altered chromatin organization in cardiomyopathic mutations of lamin A

Subhradip Nath, Shuvadip Dutta, Shreyasi Dey Sarkar, Duhita Sengupta, Mithun K Mitra, Kaushik Sengupta, Liquid–liquid phase separation of lamin drives altered chromatin organization in cardiomyopathic mutations of lamin A, Nucleic Acids Research, Volume 53, Issue 14, 12 August 2025, gkaf615, https://doi.org/10.1093/nar/gkaf615

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Defects in lamin A-Prohibitin crosstalk lead to ROS elevation and OxPhos imbalance in laminocardiomyopathy

Subhradip Nath, Soumen Kanti Manna, Debasish Prusty, Sk. Ramiz Islam, Kaushik Sengupta*

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bioRxiv 2025.07.17.665267; doi: https://doi.org/10.1101/2025.07.17.665267

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Lamins in Genome Organization and Diseases

Key highlights:

 

  • New insights into lamins in cancer, linking mutations to tumor progression and epigenetic gene regulation

  • Integration of lamin and chromatin biology, advancing our understanding of nuclear architecture in health and disease

  • Emerging directions for molecular medicine and therapeutic innovation

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To be released Sep 2025

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Liquid-liquid phase separation of lamin drives altered chromatin organisation in cardiomyopathic mutations of lamin A

Subhradip Nath, Shuvadip Dutta, Shreyasi Dey Sarkar, Duhita Sengupta, Mithun K. Mitra, Kaushik Sengupta Liquid-liquid phase separation of lamin drives altered chromatin organisation in cardiomyopathic mutations of lamin A

Accepted in OUP Nucleic Acids Research

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A System for Carrying Out Active Microrheology to Probe Viscoelasticity of Protein
Indian Patent No. 539208 (27-May-2022)

In collaboration with Dr. Ayan Banerjee’s laboratory at the Physics Department, IISER, Kolkata, we developed a novel active microrheology-based method using pulsed optical tweezers (Soft Matter, Jul 2021;17(28):6787-6796. doi: 10.1039/d1sm00293g). This method determines the viscoelasticity of lamin A protein and its mutants in vitro, corroborating the significant alterations in nuclear plasticity in the cell as a disease phenotype. The method is fast, robust, and highly reproducible, requiring minimal sample amounts, making it suitable for translation into a biological toolkit.

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Lamin A K97E leads to NF-κB-mediated dysfunction of inflammatory responses in dilated cardiomyopathy

Sengupta, D. & Sengupta, K. Lamin A K97E leads to NF-κB-mediated dysfunction of inflammatory responses in dilated cardiomyopathy. Biol. Cell 1–21 (2024) doi:10.1111/boc.202300094.

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Elevated Levels of Lamin A Promote HR and NHEJ-Mediated Repair Mechanisms in High-Grade Ovarian Serous Carcinoma Cell Line

Duhita Sengupta et. al. Elevated Levels of Lamin A Promote HR and NHEJ-Mediated Repair Mechanisms in High-Grade Ovarian Serous Carcinoma Cell Line Cells 2023, 12(5), 757 (2023)

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Slower diffusion and anomalous association of R453W lamin A protein alter nuclear architecture in AD-EDMD

Mukherjee, C. et al. Slower diffusion and anomalous association of R453W lamin A protein alter nuclear architecture in AD-EDMD. RSC Adv. 12, 32129–32141 (2022).

Lamin A and telomere maintenance in aging: Two to Tango

Sengupta, D. & Sengupta, K. Lamin A and telomere maintenance in aging: Two to Tango. Mutat. Res. Mol. Mech. Mutagen. 825, 111788 (2022).

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Active microrheology using pulsed optical tweezers to probe viscoelasticity of lamin A

Mukherjee, C., Kundu, A., Dey, R., Banerjee, A. & Sengupta, K. Active microrheology using pulsed optical tweezers to probe viscoelasticity of lamin A. Soft Matter 17, 6787–6796 (2021).

A deep hybrid learning pipeline for accurate diagnosis of ovarian cancer based on nuclear morphology

Sengupta, D., et. al. A deep hybrid learning pipeline for accurate diagnosis of ovarian cancer based on nuclear morphology Plos One. (2022).

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Pulling the springs of a cell by single-molecule force spectroscopy

Mukherjee, C. et al. Pulling the springs of a cell by single-molecule force spectroscopy. Emerg. Top. Life Sci. 5, 77–87 (2021).

Nuclear filaments: role in chromosomal positioning and gene expression

Bera, M. & Sengupta, K. Nuclear filaments: role in chromosomal positioning and gene expression. Nucleus 11, 99–110 (2020).

Emerging roles of lamins and DNA damage repair mechanisms in ovarian cancer

Sengupta, D., Mukhopadhyay, A. & Sengupta, K. Emerging roles of lamins and DNA damage repair mechanisms in ovarian cancer. Biochem. Soc. Trans. 48, 2317–2333 (2020).

Skeletal Muscle Dystrophy mutant of lamin A alters the structure and dynamics of the Ig fold domain

Dutta, S. et al. Skeletal Muscle Dystrophy mutant of lamin A alters the structure and dynamics of the Ig fold domain. Sci. Rep. 8, 1–16 (2018).

Changes in the Nuclear Envelope in Laminopathies

Dutta, S., Bhattacharyya, M. & Sengupta, K. Changes in the nuclear envelope in laminopathies. Adv. Exp. Med. Biol. 1112, 31–38 (2018).

DCM associated LMNA mutations cause distortions in lamina structure and assembly

Bhattacharjee, P., Dasgupta, D. & Sengupta, K. DCM associated LMNA mutations cause distortions in lamina structure and assembly. Biochim. Biophys. Acta - Gen. Subj. 1861, 2598–2608 (2017).

Implications and Assessment of the Elastic Behavior of Lamins in Laminopathies

Dutta, S., Bhattacharyya, M. & Sengupta, K. Implications and assessment of the elastic behavior of lamins in laminopathies. Cells 5, 1–10 (2016).

Significance of 1B and 2B domains in modulating elastic properties of lamin A

Bera, M., Ainavarapu, S. R. K. & Sengupta, K. Significance of 1B and 2B domains in modulating elastic properties of lamin A. Sci. Rep. 6, 1–12 (2016).

Molecular Events in Lamin B1 Homopolymerization: A Biophysical Characterization

Bhattacharjee, P., Dasgupta, D. & Sengupta, K. Molecular Events in Lamin B1 Homopolymerization: A Biophysical Characterization. J. Phys. Chem. B 119, 14014–14021 (2015).

Novel linkage of LMNA Single Nucleotide Polymorphism with Dilated Cardiomyopathy in an Indian case study

Banerjee, A., Ghoshal, P. K. & Sengupta, K. Novel linkage of LMNA Single Nucleotide Polymorphism with Dilated Cardiomyopathy in an Indian case study. IJC Hear. Vasc. 7, 99–105 (2015).

Characterization of Unfolding Mechanism of Human Lamin A Ig Fold by Single-Molecule Force Spectroscopy—Implications in EDMD

Bera, M. et al. Characterization of unfolding mechanism of human lamin A Ig fold by single-molecule force spectroscopy-implications in EDMD. Biochemistry 53, 7247–7258 (2014).

Viscoelastic Behavior of Human Lamin A Proteins in the Context of Dilated Cardiomyopathy

Banerjee, A. et al. Viscoelastic behavior of human lamin A proteins in the context of dilated cardiomyopathy. PLoS One 8, (2013).

Structural Alterations of Lamin A Protein in Dilated Cardiomyopathy

Bhattacharjee, P., Banerjee, A., Banerjee, A., Dasgupta, D. & Sengupta, K. Structural Alterations of Lamin A Protein in Dilated Cardiomyopathy. Biochemistry 52, 4229–4241 (2013).

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